FIG. 1.

PERV transmission to primary fibroblasts of different primate species. Primary human foreskin fibroblasts (HFF) and gorilla (GGO205), baboon (P. hamadryas, PHA421), M. nemestrina (M.nem.), and M. fascicularis (M.fasc.) skin fibroblasts were infected by cocultivation (cc) with lethally irradiated PK15 or PAE cells and by cell-free supernatant (sup), respectively, and analyzed for PERV transmission by PERV env-specific PCR. False-positive results due to amplification of PERV sequences from contaminating porcine DNA were excluded by pig cytochrome b-specific PCR (pig CytB). Mammalian cytochrome b-specific PCR (CytB) was the positive control.