Extended Data Fig. 6 ∣. Spine-mounted macroscopes of <10 g and low center of gravity have little effect on mouse open-field behavior.

a,e,g, Schematics of the open-field test and analysis approach used to compare the animal’s general locomotor activity with or without the ~9.8 g macroscope mounted to its lumbar spinal cord following a 3–5 d habituation period. b–d,f,h, Population data from all animals (N = 5) and analysis periods (six consecutive 5-min recordings per animal) (left) or only the first and last five minutes (right) of the 35–40 min recordings for each condition (mounted, unmounted) (Methods). Evaluated parameters included the animal’s total distance traveled (b), average running speed (c), rearing activity (d), inner versus outer zone occupancy (f), and quadrant/zone 1–4 occupancy (h). Paired two-sided t-tests determined P values (b, left: 0.1226; b, right: 0.0944, 0.0814; c, left: 0.1465; c, right: 0.5371, 0.3636; d, left: 0.1214; d, right: 0.6317, 0.3271; f, left: 0.0158, 0.1456, 0.2056, 0.0146; f, right: 0.8970, 0.7451, 0.7738, 0.7166; h, left: 0.6676, 0.7382, 0.7114, 0.7215; h, right: 0.5705, 0.8128, 0.6599, 0.8191, 0.1716, 0.6295, 0.2200, 0.2402). All data are presented as mean ± s.e.m.