Fig. 2.

Hepatic CGL gene and protein expression are upregulated in MR mice. A Gene expression was determined by quantitative real-time PCR (qPCR) using TAQMAN primers normalized to β-actin, as described in the “Methods” section (**P < 0.01, n = 7–8/group). B Protein expression was determined by Western blot as described in the “Methods” section. Bands represent proteins from liver tissue samples of control-fed (CF) or methionine-restricted (MR) mice. C Band intensities (**P < 0.001, n = 7/group) were quantified as described in the “Methods” section