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. 2023 May 23;46(4):3617–3634. doi: 10.1007/s11357-023-00788-4

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© The Author(s) 2023

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Fig. 5 — Cystine is a CGL substrate. Here, the kinetics of CGL-catalyzed 2-oxobutanoate production using cystine as a substrate is shown. The data for this study was obtained as described in the “Methods” section using 360 ng rat liver CGL (8.26 pmol) in a reaction volume of 50 µL. A The data as function of cystine concentrations; B the double reciprocal plot of the same data. The data are depicted as the average ± SD of triplicate determinations, while the plots were fitted using Prism software. In both cases, the plots have R.² values of 1.00