Table 3. Data collection and processing.
Values in parentheses are for the outer shell.
| Fragment antibody | D08 | D08* | BLV5B8* |
|---|---|---|---|
| Diffraction source | Beamline I04, Diamond Light Source | ||
| Wavelength (Å) | 0.9795 | ||
| Temperature (K) | 100 | ||
| Detector | Dectris EIGER X 16M pixel | ||
| Rotation range per image (°) | 0.1 | 0.1 | 0.1 |
| Total rotation range (°) | 180.0 | 180.0 | 360.0 |
| Exposure time per image (s) | 0.008 | 0.008 | 0.008 |
| Space group | P212121 | P212121 | C121 |
| a, b, c (Å) | 63.5, 71.1, 98.4 | 65.0, 70.9, 96.0 | 161.2, 49.0, 60.4 |
| α, β, γ (°) | 90.0, 90.0, 90.0 | 90.0, 90.0, 90.0 | 90.0, 90.9, 90.0 |
| Resolution range (Å) | 57.64–1.59 (1.62–1.59) | 39.74–1.45 (1.48–1.45) | 80.57–1.46 (1.50–1.46) |
| Total No. of reflections | 408647 (20025) | 526665 (22235) | 539903 (23229) |
| No. of unique reflections | 60540 (3079) | 79276 (3919) | 82095 (5807) |
| Completeness (%) | 100.0 (100.0) | 100.0 (100.0) | 100.0 (100.0) |
| Multiplicity | 6.7 (6.8) | 6.6 (5.7) | 6.6 (5.7) |
| 〈I/σ(I)〉 | 14.0 (1.3) | 10.7 (0.5) | 12.0 (0.4) |
| R r.i.m. | 0.063 (1.416) | 0.076 (1.946) | 0.076 (2.019) |
| R p.i.m. | 0.024 (0.710) | 0.029 (0.806) | 0.029 (0.847) |
| Overall B factor from Wilson plot (Å2) | 27 | 24 | 24 |