Skip to main content
. 2024 Jun 27;80(Pt 7):135–141. doi: 10.1107/S2053230X24005831

Table 2. Crystallization conditions of diffracting crystals.

Method Vapor diffusion in hanging drops
Temperature (K) 294
Protein concentration (mg ml−1) 11.5
Buffer composition of protein solution 50 mM Tris–HCl pH 8.0, 150 mM NaCl, 10% glycerol
Composition of reservoir solution 0.1 M sodium citrate pH 5.0, 6%(v/v) 2-propanol, 16%(w/v) PEG 400
Volume and ratio of drop 3 µl, 1.5:1.5 ratio
Volume of reservoir (µl) 300