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. 2024 Jun 27;13(1):2373317. doi: 10.1080/22221751.2024.2373317

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© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 4. — TmytC is a virulence factor of T. pulmonis. (a) Gross appearance of the rabbit eyes after intrastromal injection of the PW1004-WT and its derivative mutants. The symptoms of keratitis in rabbits infected with PW1004ΔtmytC appeared less severe with fewer purulent discharges compared to those infected with the PW1004-WT and PW1004ΔtmytC/ptmytC mutant. (b) Representative images of H&E staining of corneal sections of infected rabbits. PMN (shown in blue arrows) infiltration in corneal stroma and stromal oedema was less prominent in PW1004ΔtmytC compared to the PW1004-WT and PW1004ΔtmytC/ptmytC mutants, showing the disorderly arranged epithelial layer. (c) Mean bacterial load in cornea inoculated with the PW1004-WT, PW1004ΔtmytC, and PW1004ΔtmytC/ptmytC at day 2 PI. Error bars indicate means ± SEM of three independent experiments.