Figure 1. The 12-LOX pathway, stimulated by PLY-producing Sp, promotes PMN infiltration, lung permeability and bacteremia following Sp lung infection in mice.

BALB/c mice were infected i.t. with 1 × 10⁷ CFU wild type (WT) or PLY-deficient mutant (Δply) TIGR4 Sp for 18 h, with or without i.p injection of 8 mg/kg of the 12-LOX inhibitor CDC. (a) Bacterial lung burden determined by measuring CFU in lung homogenates. (b) PMN infiltration determined by flow cytometric enumeration of Ly6G⁺. (c) Lung permeability quantitated by measuring the concentration of 70 kDa FITC-dextran in the lung relative to serum after i.v. administration. (d) Bacteremia measured by enumerating CFU in serum. Each panel is representative of three independent experiments, or pooled data from three independent experiments. Error bars represent mean ± SEM. Statistical analysis was performed using ordinary one-way ANOVA: *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001, ****p-value < 0.0001.