FIG. 3.

Nef protein expression as detected by Western blots. Vero cells were infected with the indicated viruses or mock infected. Cells were harvested at 9 or 12 hpi, and lysates were resolved by SDS-PAGE. Nef protein was detected following incubation with the N27 anti-Nef monoclonal antibody in K81- and d81-infected cell lysates as well as in the Nef-positive control lysate, which migrated slower due to an epitope tag. Nef protein was not detected in cells infected with TK⁻ HSV, in mock-infected cells, or in nef⁻ lysates.