Table 3.

Intra-person variability was evaluated for three different investigators. Each investigator prepared three separate curves. Protein concentrations were calculated for hypothetical absorbance values using each curve.

Hypothetical Sample (A565)	Trial 1	Trial 2	Trial 3	Ave	Std Dev	CV
Investigator 1
0.10	9.61	8.11	5.63	7.78	2.01	0.26
0.20	68.44	66.21	68.82	67.82	1.41	0.02
0.30	129.25	126.90	133.06	129.74	3.11	0.02
0.40	192.24	190.55	198.41	193.73	4.14	0.02
0.50	257.66	257.66	264.93	260.08	4.19	0.02
0.60	325.84	328.85	332.67	329.12	3.43	0.01
0.70	397.13	404.97	401.72	401.27	3.94	0.01
0.80	472.02	487.21	472.14	477.13	8.73	0.02
Investigator 2
0.10	6.36	7.23	4.23	5.94	1.54	0.26
0.20	69.51	68.96	76.14	71.54	4.00	0.06
0.30	131.49	130.86	148.56	136.97	10.04	0.07
0.40	192.36	192.92	221.48	202.25	16.66	0.08
0.50	252.18	255.14	294.93	267.42	23.87	0.09
0.60	311.00	317.53	368.91	332.48	31.72	0.10
0.70	368.87	380.09	443.44	397.46	40.21	0.10
0.80	425.83	442.81	518.53	462.39	49.35	0.11
Investigator 3
0.10	1.57	NA	NA	1.57	NA	NA
0.20	52.38	38.56	47.32	46.08	6.99	0.15
0.30	104.99	79.73	97.21	93.97	12.94	0.14
0.40	159.61	122.02	148.33	143.32	19.29	0.13
0.50	216.49	165.54	200.77	194.27	26.09	0.13
0.60	275.94	210.40	254.63	246.99	33.43	0.14
0.70	338.35	256.73	310.05	301.71	41.44	0.14
0.80	404.20	304.69	367.16	358.68	50.30	0.14