Figure 2:

The impact of T6I-29-1A on the proliferation and ER target gene expression of Y537S ESR1 mutant breast cancer cells. A) T47D Y537S ESR1 and B) MCF7 Y537S ESR1 breast cancer cell proliferation, treated with 1 μM compound in the presence of 1 nM E2. Graphs represent mean of three independent replicates, data normalized to E2 treatment, error bars are s.d. Statistical analysis was performed using ANOVA with Tukey post-hoc test. C-F) RT-qPCR in T47D Y537S ESR1 and G-J) MCF7 Y537S ESR1 cells. Representative data are the mean of three replicates ± s.d. and error bars show s.d. Significance determined by one-way ANOVA test with tukey post-hoc where *p < 0.05, **p < 0.005, ***p < 0.0005, and ****p < 0.00005.