Extended Data Table 1.

Michaelis-Menten kinetics for alanine scanning mutants of mini-cAMPase

Kinetics were measured at 50 µM enzyme concentration in 50 mM HEPES-NaOH, 150 mM NaCl, 200 µM MnCl₂, pH 8.0 at 25 °C. Note that for truncated proteins, which form dimers, the kinetics are calculated per protein dimer.