FIG. 5.

Localization of the attB site on the physical and genetic maps of the S. pneumoniae M24 DNA. (A) PFGE of the DNA obtained from strain M24 digested with ApaI, SacII, or SmaI was performed, and the fragments were blotted and hybridized with a DNA probe containing the attB site (see text). (B) The localizations of most restriction fragments and the genetic markers are taken from reference (25), and the attB site is shown in boldface. At the bottom, the location of attB is denoted by a hatched flag in contig sp_100 as deduced from the preliminary sequence of the genome of S. pneumoniae already released. A fragment of contig sp_29 located upstream of sp_100 is also shown. ORFs that have been described in previous publications are designated by their gene names, and the rest of the genes are identified by the designation of their most similar homologues.