FIG. 2.

Cell killing by various adenovirus E4orf4 proteins. H1299 cells were lipofected with plasmid DNA expressing neo and wild-type or mutant E4orf4 and then trypsinized, replated, and allowed to grow in the presence of G418. The results represent the averages of two separate experiments, each involving three plates per sample. The standard error in such experiments was less than 30% (top). At the time of replating, some cells were examined for E4orf4 expression by Western blotting using anti-HA antibodies (bottom).