Figure 3.

Inducible cardiomyocyte-specific acetyl-CoA carboxylase-1/2 knockout mice.A, inducible cardiomyocyte-specific ACC-1/2 knockout mice (αMHC-MerCreMer cross with Acc1^f/f/Acc2^f/f) were generated as indicated. B, three days following the final tamoxifen injection (3 injections every other day of 0.5 mg in 50 μl 10% v/v ethanol/sesame oil) hearts were harvested from control and ACC-1/2 knockout mice and total cardiac ACC protein quantified by Western blot followed by densitometric analysis (n = 6). C, control, and ACC KO mice were injected with insulin (0.05 U/g) intraperitoneally at 8 AM. Hearts were then excised and frozen 10 min post-injection. Heart homogenate was centrifuged (500g, 5 min, 4 °C) and the supernatant (S1) evaluated for (C) total AKT and phosphorylation status of the Thr³⁰⁸ site on AKT and (D) lipoic acid conjugated to PDH E2 or α-ketoglutarate dehydrogenase (KGDH) E2 and total PDH E1 by Western blot analysis followed by densitometric analysis. Values are presented as the mean ± SD (n = 3–6) where significant differences (2-tailed t test or in experiments with multiple variables by ANOVA with the Tukey test) are indicated by ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. Each data point indicates a separate animal.