Fig. 8.

Exosomal let-7b-5p suppress microglia pyroptosis by regulating LRIG3 expression. (A) Exosomal let-7b-5p regulates LRIG3 by directly targeting the 3′-UTR; (B) Luciferase report assay was performed to confirm LRIG3 is the target gene of let-7b-5p; (C) Immunoprecipitation of the Ago2/RISC (RNA-induced silencing complex) using the Pan-Ago2 antibody in BV2 microglia overexpressing miR-NC or let-7b-5p. IgG was used as a negative control and GAPDH was used as an internal control; (D) The mRNA level of LRIG3 in BV2 cells after treatment with let-7b-5p^OE-Exos and let-7b-5p^KD-Exos; (E) The protein level of LRIG3 in BV2 cells after treatment with let-7b-5p^OE-Exos and let-7b-5p^KD-Exos; (F) Quantitative analysis of LRIG3 protein; (G-J) Rescue experiments for let-7b-5p inhibition were conducted by downregulating LRIG3 in microglia. Microglia pyroptosis was detected by western blot analysis and immunofuorescence; (K-N) Rescue experiments for let-7b-5p overexpression were carried out by the ectopic expression of LRIG3 in microglia. Microglia pyroptosis was detected by western blot analysis and immunofuorescence. *P < 0.05; **P < 0.01; ***P < 0.001 (n = 3 per group)