Fig. 4.

Yard. 2 modulates HIF-1 target genes and acts as a microtubule inhibitor. PC3 cells were treated in normoxia (Nx) and hypoxia (Hx 1%) in the absence or presence of Yard. 2 at 20μM for 48h. A Venn diagram showing the differential distribution of the genes detected between hypoxia and normoxia (in blue) and between Hx + Yard. 2 and Nx (in red). B Gene set enrichment list of RNA-Seq data comparing hypoxia (Hx) and normoxia (Nx) using “Cellular functions”. C Gene set enrichment list of RNA-Seq data comparing hypoxia + Yard. 2 (Hx + Yard. 2) and normoxia (Nx) using “Cellular functions”. D and E Heatmap (D) and the representative bar diagram (E) of some specific HIF-1 target genes in normoxia (Nx) compared to hypoxia (Hx) or hypoxia treated with Yard. 2. F PCA plot of hypoxia (Hx) and hypoxia + Yard.2 (Hx + Yard. 2) samples. Ellipses and shapes show clustering of the samples. G gene set enrichment map of RNA-Seq data comparing hypoxia + Yard. 2 (Hx + Yard. 2) and hypoxia (Hx) using “Cellular components”. H Gene set enrichment list of RNA-Seq data comparing hypoxia + Yard. 2 (Hx + Yard. 2) and hypoxia (Hx) using “Molecular functions”