Fig 1.

Two-dimensional plot of the quadruplex ddPCR assay. Sixteen clusters of droplets (maximum possible) represent (1): droplets containing no target DNA (negative population), (2): droplets containing at least one copy of pfhsp70, (3): droplets containing at least one copy of pfmdr1, (4): droplets containing at least one copy of pfpmp3, (5): droplets containing at least one copy of pfpmp2, (6): droplets with both pfpmp3 and pfmdr1, (7): droplets with both pfpmp3 and pfhsp70, (8): droplets with both pfmdr1 and pfhsp70, (9): droplets with both pfhsp70 and pfpmp2, (10): droplets with pfmdr1, pfhsp70, and pfpmp3, (11): droplets with pfpmp2 and pfmdr1, (12): droplets with pfhsp70, pfmdr1, and pfpmp2, (13): droplets with pfpmp2 and pfpmp3, (14): droplets with pfhsp70, pfpmp2, and pfpmp3, (15): droplets with pfmdr1, pfpmp2, and pfpmp3, (16): and droplets with pfmdr1, pfpmp2, pfpmp3, and pfhsp70.