TABLE 1.

Optimized quadruplex assay reaction primer and probe concentrations^a

Target	Probe concentration (nm)	Fluorophore	Primer concentration (nm)
pfpmp2a	125	HEX	2,500
pfhsp70b	125	HEX	800
pfpmp3a	50	FAM	400
pfmdr1	125	FAM	600

^a Bold targets indicate assays with higher fluorescence amplitudes on ddPCR plots.

^b Used as a single-copy reference gene.