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Characterization of mIL-4-expressing CVB3 strains in cell culture. (A) Single-step growth curves of the viruses on HeLa cells. (B) Concentration of mIL-4 in lysates as measured by ELISA. HeLa cells were inoculated with the parental virus CVB3/0 or the chimeric strains CVB3/0-mIL4-47 and CVB3-PL2-mIL4-46. Inoculated cell cultures were harvested by freeze-thaw lysis at the stated times. Error bars indicate standard error of the mean.
