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. Author manuscript; available in PMC: 2024 Jul 9.
Published in final edited form as: J Vis Exp. 2023 May 16;(195):10.3791/65287. doi: 10.3791/65287

Table 1: Troubleshooting table.

Several typical problems, their possible causes, and suggested remedial actions are provided.

Name of Material/ Equipment Company Catalog Number Comments/Description
5X Magnifying Glass with LED Light and Stand amazon.com B08QJ6J8P1 light must not produce heat
Disposable Transfer Pipets VWR 414004-036
Dissecting Fine-Pointed Forceps Fisher Scinetific 08-875
Dissecting scissors sharp piont, straight 6.5” VWR 76457-374
Dissection Tray Fisher Scinetific 14-370-284 styrofoam sheets are an acceptable alternative
Fine Dissection Pins Living Systems Instrumentation PIN-#3
General Use Hypodermic Needles, 22 gauge Fisher Scientific 14-826-5A
General Use Hypodermic Needles, 25 gauge Fisher Scientific 14-826AA
Heparin, Porcine Intestinal Mucosa MilliporeSigma 37-505-410MG
Iridectomy Scissors 6” vwr 470018-938 iris scissors are an acceptable alternative
Luer-to-Barb Adapter Male Luer with Lock Ring amazon.com B09PTX6M2Z size will be dependant on the hosing of the pump used
Mayo-Hegar Needle Holder Fisher Scinetific 08-966 mosquito forceps are an acceptable alternative
MS-222: Syncaine (formerly tricaine) Pentair AES TRS1
PBS x 1 Corning 21-040-CV
Peristaltic Liquid Pump Dosing Pump 5-100mL/min amazon.com B07PWY4SM6 any peristaltic pump capable of pumping 5-10mL/min is acceptable
Sodium Bicarbonate, Powder, USP Fisher Scientific 18-606-333
Specimen Forceps, Serrated VWR 82027-442
T-Pins for Dissecting Fisher Scinetific S99385
Ultra-Fine Short Insulin Syringes, 31 gauge VWR BD328438
Wire Flush Cutters, 6-inch Ultra Sharp & Powerful Side Cutter Clippers amazon.com B087P191LP