Table II.
Additional microorganisms and new species detected by BioFire Joint Infection panel technology for each discordant synovial fluid sample. Discrepant results were noted in culture-negative cases, monomicrobial infected cases, and single-intraoperative culture cases. All 16 cases with discordant results were confirmed by specific microorganism polymerase chain reaction followed by sequencing.
| Result | Conventional microbiology | JI panel | ICM-2018 classification |
|---|---|---|---|
| Negative to positive microbiological result | |||
| 1 | None | Streptococcus spp | Infected |
| 2 | None | Streptococcus spp | Infected |
| 3 | None | Streptococcus agalactiae | Infected |
| 4 | None | Staphylococcus lugdunensis | Infected |
| 5 | None | Staphylococcus lugdunensis | Infected |
| 6 | None | Staphylococcus aureus (MSSA) | Non-infected |
| 7 | None | Candida albicans | Inconclusive |
| 8 | None | Staphylococcus aureus (MSSA) | Infected |
| 9 | None | Streptococcus spp | Inconclusive |
| 10 | None | Streptococcus agalactiae | Infected |
| Monomicrobial with additional microbiological result(s) | |||
| 1 | Staphylococcus aureus (MSSA) | Staphylococcus aureus (MSSA), Enterococcus faecium (VRE) | Infected |
| 2 | Proteus mirabilis | Proteus spp, Staphylococcus aureus (MSSA), Anaerococcus prevotii, Finegoldia magna, Peptoniphilus | Infected |
| 3 | Enterococcus faecalis (VSE) | Enterococcus faecalis (VSE), Fineglodia magna, Peptoniphilus, Streptococcus spp | Infected |
| 4 | Candida albicans | Candida albicans, Staphylococcus aureus (MSSA) | Infected |
| 5 | Staphylococcus aureus (MSSA) | Staphylococcus aureus (MSSA), Proteus spp | Infected |
| SPC in sonication fluid with additional microorganism | |||
| 1 | Staphylococcus capitis | Staphylococcus aureus (MSSA) | Infected |
ICM, International Consensus Meeting; JI, joint infection; MSSA, methicillin-susceptible Staphylococcus aureus; SPC, single-positive culture; VRE, vancomycin-resistant Enterococcus; VSE, vancomycin-susceptible Enterococcus.