Fig. 3.

Fluorescence microscopy images of SV-synapsin condensates for different protein-to-lipid ratios and a phase diagram for the condensate formation of synapsin and SV. a Fluorescence microscopy image of condensates consisting of $6\mathrm{\mu }\text{M}$ synapsin and $10\text{nM}$ SVs ($P/L=1:12$). Small spherical condensates are visible. b Fluorescence microscopy image of condensates consisting of $6\mathrm{\mu }\text{M}$ synapsin and $35\text{nM}$ SVs ($P/L=1:41$). The lower P/L leads to the formation of fractal-like condensates. c Fluorescence microscopy image of condensates consisting of $0.66\mathrm{\mu }\text{M}$ synapsin and $35\text{nM}$ SVs ($P/L=1:373$). All images were taken at a magnification of 20$\times$ and 20–30 min after mixing. d Phase diagram for the formation of condensates consisting of SVs and synapsin. Circles indicate the formation of spherical condensates, the ’fractal symbol’ indicates the formation of fractal condensates. Fluorescence microscopy images for all concentrations indicated in the diagram are shown in Fig. S3 in the supplementary material. Please note, that the more diffuse fluorescent signal is caused by unfocussed isolated condensates and does not stem from connections between clusters