Fig. 4.

Cryo-EM micrographs of LV4 taken at a magnification of 8.7 kx. a LV4 (extrusion through 50 nm pore membrane, lipid concentration of 10 mM) in ultrapure water. The extrusion step in the preparation leads to an alteration of the size distribution of the vesicles. b LV4 (membrane pore size 50 nm, lipid concentration 15 mM) in buffer. The buffer has a significant influence on the vesicle shape. c Segmentation of LV4 (exemplary shown in (a)). d Distribution fit of LV4 radii resulting from the segmentation. The total number of segmented vesicles is 586 and the lognormal fit results in a mean of 41.3 nm with a standard deviation of 10.8 nm. It should be noted that the vesicle radii are slightly larger because the outer edge of the vesicles was not detected during segmentation