Fig. 7.

ZBTB7A overexpression dramatically reduced the angiogenesis, metastasis, and proliferation of EC cells in vivo (A and B) In vivo overexpression of pcDNA-ZBTB7A in HEC-1 A and KLE cells resulted in considerably lower tumour weight and volume compared to the sh-NC-transfected control group. (C, D, and E) The frequencies of cells that were positive for Ki67, CD31, and VEGFA significantly decreased, according to immunohistochemistry. (F) HE staining revealed that there were considerably less HEC-1 A and KLE cell-produced nodules in mouse lung tissue. Data from at least three replicates were presented as mean ± standard deviation (SD). *P < 0.05, **P < 0.01