Table 1.
Primer sequences of genes detected by ddPCR.
| Gene | F/R | Sequence | Tm (°C) |
|---|---|---|---|
| SPAST | F | TTTTCCAAGTCACAAACGGACG | 55.5 |
| R | ATTGCGGCATGCCAAGTTAG | 56.1 | |
| CDKN2A | F | GTGCGCAGGGCTCAGA | 58.9 |
| R | CCACCCGGACCTCCAAG | 58.9 | |
| CDKN2B | F | CGCATGCGTCCTAGCATC | 56.8 |
| R | GGGGAAGCCTGCCCAA | 58.9 | |
| CDK4 | F | TTCCCATCAGCACAGTTCGT | 56.4 |
| R | TCCAGTCGCCTCAGTAAAGC | 57 | |
| E2F1 | F | GCTACCGGGAACGATTCCAT | 57.2 |
| R | AGGCCTGGAGTTGTGTTGAA | 56.4 | |
| TFG | F | GGCGCCTACGGGAATAAGTT | 57.5 |
| R | CCAAGGAACTAGGCTCCGAT | 56.4 | |
| KIF25 | F | ATAGAGACCAGACGGAGGCT | 57.1 |
| R | ACCAAGTGGGTGTCATAGGC | 57.2 | |
| DIP2C | F | AGGCTTACTGCTGACTGCAT | 56.2 |
| R | TATGTTGTGGGCAGCGTCTT | 56.7 |