FIG. 6.

SFV-expressed VP7 requires ATP for correct folding. MA-104 cells were infected with recombinant SFV VP7 (MOI, 10). At 18 h p.i., the cells were starved for 1 h in methionine- and cysteine-free medium and subsequently labeled with [³⁵S]methionine (250 μCi) for 15 min. DTT (2 mM) was added to the medium at 18.5 h p.i. and maintained during the pulse. The cells were chased in oxidizing ATP (+ATP) or ATP-deficient (−ATP) medium for 60 min. The monolayers were subsequently incubated with ice-cold PBS-NEM, harvested in lysis buffer, immunoprecipitated with M60, and analyzed by reducing SDS-PAGE. Molecular mass markers (in kilodaltons) are shown on the right side of the panel.