Table 2.
Sequence of primers for real-time PCR.
| Target gene | Primer sequence (5′–3′) | Product size (bp) | Accession number |
|---|---|---|---|
| TNF-α | Forward: ACCATGGAGTCCTTCAGCTC | 144 | NC_067386.1 |
| Reverse: TTTGGACACACGGTAGAGCT | |||
| IL-10 | Forward: AAACAAGAGCAAGGCAGTGG | 170 | NC_067389.1 |
| Reverse: GGATGGAGTTCTCCTGGCTT | |||
| TLR4 | Forward: TGCCACCTGTCAGATAAGCA | 121 | NC_067374.1 |
| Reverse: TCGGCCAACTAGAAGCATCA | |||
| MyD88 | Forward: AGACCAACTACCGGCTGAAG | 171 | NW_026259666.1 |
| Reverse: GGGCAAACTTGGTCTGGAAG | |||
| NF-κB | Forward: CAACCCACCTACCCATCTGT | 123 | NC_067395.1 |
| Reverse: GCACACGGGGTTTGAAGAAT | |||
| IL-β | Forward: TGTAGACCCCAACCGTTACC | 149 | NC_067375.1 |
| Reverse: GGAAGACGGGCATGTACTCT | |||
| IL-6 | Forward: GCAGAACCATCGAGAGCATC | 178 | NC_067383.1 |
| Reverse: GTCTCATTATTCACCGCCGG | |||
| IkBα | Forward: TGCACTTGGCCATCATCCAT | 190 | NM_020529.3 |
| Reverse: TCTCGGAGCTCAGGATCACA | |||
| Occludin | Forward: ATTTTGACACTGGCCTGCAG | 143 | NC_067384.1 |
| Reverse: GTTGTACTCATCAGCAGCCG | |||
| ZO-1 | Forward: CCGTGTTGTGGATACCTTG | 89 | NC_067390.1 |
| Reverse: TGCCTCGTTCTACCTCCTT | |||
| Claudins-1 | Forward: AGCATGGTATGGCAACAGA | 121 | NC_067387.1 |
| Reverse: CAGAAGGCAGAGAGAAGCA | |||
| β-actin | Forward: CCTGGCACCCAGCACAAT | 118 | NW_026259299.1 |
| Reverse: GCTGATCCACATCTGCTGGAA |