FIG. 9.

Effects of HNF-3α and HNF-3β on enJSRV LTRs. pJS21-luc, penJS56A1-luc, penJS5F16-luc, and penJS59A1-luc were cotransfected into NIH 3T3 cells (that do not efficiently support JSRV enhancer activity) along with an expression plasmid for either HNF-3α or HNF-3β. Different amounts of the transcription factor expression plasmids were cotransfected with a set amount (200 ng) of the reporter plasmid DNA. The amounts of luciferase activity for the different cotransfections are shown as fold activation over the activity of reporter plasmid cotransfected with a plasmid having the CMV promoter but not HNF-3 insert.