Figure 2.

KIF22 is essential for cell proliferation in ATDC5 cells

ATDC5 cells were infected with control shRNA adenovirus (shcontrol), Kif22 shRNA adenovirus (shKif22), and/or Myc-tagged-Kif22 overexpression adenovirus (Kif22).

(A) Kif22 expression was examined by western blotting analysis. Uncropped image was shown in Figure S1.

(B) ATDC5 cells were plated at 3,000 cells/cm² in 96-well plates. CCK8 cell viability assay showing proliferation of ATDC5 cells. Data are represented as mean ± SD; n = 4. ∗; p < 0.05. Other statical data were shown in Table S1.

(C) Ki67 immunofluorescence staining and quantification 48 h after infection. Scale bar, 50 μm. Data are represented as mean ± SD; n = 3. ∗; p < 0.05. Other statical data were shown in Table S1.

(D) KIF22 and tubulin immunostaining 24 h after infection and cell synchronization. Nuclei were counterstained with DAPI. Mitotic nuclei (arrows) were quantified. Scale bar, 10 μm. Data are represented as mean ± SD; n = 4. ∗; p < 0.05. Other statical data were shown in Table S1.

(E and F) Alcian blue staining (E) and qPCR analysis (F), 7 days after induction of differentiation. Data are represented as mean ± SD; n = 4. ∗; p < 0.05. Other statical data were shown in Table S1. More information is available at Figure S1.