REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-KIF22 antibody	GeneTex	Cat#GTX66353; RRID:AB_2943604
Anti-Ki67 antibody	Abcam	Cat#ab15580; RRID:AB_443209
Anti-cleaved caspase3 antibody	Cell signaling technologies	Cat#9664; RRID:AB_2070042
Anti-α-tubulin antibody	Fujifilm Wako	Cat#017–25031; RRID:AB_2943605
Anti-Myc-tag antibody	MEDICAL & BIOLOGICAL LABORATORIES CO., LTD.	Cat#M192-3; RRID:AB_11160947
Anti-GAPDH, HRP conjugate	MEDICAL & BIOLOGICAL LABORATORIES CO., LTD.	Cat#M171-7; RRID:AB_10699462
Anti-Mouse, HRP conjugate	Jackson ImmunoResearch Laboratories	Cat#115-035-062; RRID:AB_2338504
Anti-Rabbit, HRP conjugate	Jackson ImmunoResearch Laboratories	Cat#111-035-045; RRID:AB_2337938
Alexa Fluor 546-conjugated anti-rabbit IgG	Thermo Fisher Scientific	Cat#A-11035; RRID:AB_2534093
Alexa Fluor 488-conjugated anti-rabbit IgG	Thermo Fisher Scientific	Cat#A-32731; RRID:AB_2633280
Alexa Fluor 555-conjugated anti-mouse IgG	Thermo Fisher Scientific	Cat#A-32727; RRID:AB_2633276
Alexa Fluor 488-conjugated anti-mouse IgG	Thermo Fisher Scientific	Cat#A-32723; RRID:AB_2633275
Bacterial and virus strains
5-alpha Competent E. coli	New England Biolabs	Cat#C2987H
Chemicals, peptides, and recombinant proteins
Alcian blue 8GX	Merck	Cat#A5268-10G
Alizarin Red S	Fujifilm wako	Cat#011-01192
Methyl-methacrylate and 2-hydroxyethyl methacrylate mixture	Fujifilm wako	Cat#086-04385
Dulbecco’s Modified Eagle Medium (DMEM)/Ham’s F12	Fujifilm Wako	Cat#048-29785
Streptomycin	Fujifilm Wako	Cat#168-23191
Insulin-Transferrin-Selenium (ITS) solution	Thermo Fisher Scientific	Cat#41400045
DMEM	Fujifilm Wako	Cat#043-30085
Collagenase	Fujifilm Wako	Cat#034-22363
TRIzol™ Reagent	Thermo Fisher Scientific	Cat#15596026
Power Up SYBR™ Green Master Mix	Thermo Fisher Scientific	Cat#A25742
ProLong™ Glass Antifade Mountant	Thermo Fisher Scientific	Cat#P36984
4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI)	Dojindo	Cat#D212
Protease Inhibitor Cocktail SetⅠ	Fujifilm Wako	Cat#165-26021
PhosSTOP™	Merck	Cat#4906845001
Immobilon ECL Ultra Western HRP Substrate	Merck	Cat#WBULS0100
Nocodazol	Selleck Biotech	Cat#S2775
Critical commercial assays
PrimeSTAR Max	Takara Bio	Cat#R045A
PrimeSTAR Mutagenesis Basal Kit	Takara Bio	Cat#R046A
Fast Gene™ RNA Basic Kit	Nippon Gene	Cat#FG-80050
High-Capacity cDNA Reverse Transcription Kit	Thermo Fisher Scientific	Cat#4368814
Cell Counting Kit-8 (CCK8)	Dojindo	Cat#CK04
MEBSTAIN Apoptosis TUNEL Kit Direct	MBL	Cat#8445
Dual-Glo Luciferase Assay System	Promega	Cat#E2920
Nano-Glo Dual-Luciferase Reporter Assay System	Promega	Cat#N1610
Experimental models: Cell lines
ATDC5 cells	Riken Bio Resource Center Cell Bank	Cat#RCB0565; RRID:CVCL_3894
HEK293 cells	Riken Bio Resource Center Cell Bank	Cat#RCB1637; RRID:CVCL_0045
Experimental models: Organisms/strains
C57BL/6N	CLEA Japan	N/A
Kif22ΔC/C57BL/6N	This paper	N/A
Oligonucleotides
Primer: Kif22 Forward: GGCCGCTGTGTAAGCAAAG	This paper	N/A
Primer: Kif22 Reverse: TCCTTCGCTTCTGTCTCTCCA	This paper	N/A
Primer: Col2a1 Forward: GCTGGTGAAGAAGGCAAACGAG	Nakatomi et al.37	https://doi.org/10.1016/j.bone.2019.01.002
Primer: Col2a1 Reverse: CCATCTTGACCTGGGAATCCAC	Nakatomi et al.37	https://doi.org/10.1016/j.bone.2019.01.002
Primer: Acan Forward: CAGCTGCCCTTCACATGTAAA	Nakatomi et al.37	https://doi.org/10.1016/j.bone.2019.01.002
Primer: Acan Reverse: TGGACAAAGCCCTCAGTACACT	Nakatomi et al.37	https://doi.org/10.1016/j.bone.2019.01.002
Primer: Tbp Forward: GAAGCTGCGGTACAATTCCAG	Goto et al.38	https://doi.org/10.1177/00220345221075966
Primer: Tbp Reverse: CCCCTTGTACCCTTCACCAAT	Goto et al.38	https://doi.org/10.1177/00220345221075966
Primer: qPCR Kif22 wt check Forward: aacctggagtgattcctcggg	This paper	N/A
Primer: qPCR Kif22 ΔC check Forward: aacctggagtgattcctcAgC	This paper	N/A
Primer: qPCR Kif22 check Reverse: atgcaggatccaagaggtctaatac	This paper	N/A
Recombinant DNA
pBAsi-mU6 vector	Takara Bio	Cat#3222
Cosmid vector pAxcwit2	Takara Bio	Cat#6174
pcDNA3.1	Thermo Fisher Scientific	Cat#V79020
Cosmid vector pAxEFwtit2	Takara Bio	Cat#6174
pTagRFP-tubulin	Evrogen	Cat#FP145
pGL4.74[hRluc TK]	Promega	Cat#E6921
pBiT1.1-C [TK/LgBiT] Vector	Promega	Cat#N196A
pBiT2.1-C [TK/SmBiT] Vector	Promega	Cat#N197A
pGL4.53[luc2/PGK] Vector	Promega	Cat#E501A
Col2a1-luc	Hata et al.39,40
Software and algorithms
ImageJ	Schneider et al.41	https://imagej.nih.gov/ij/