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. 2024 Jun 15;105:105196. doi: 10.1016/j.ebiom.2024.105196

Fig. 2.

Fig. 2

RNAscope detects intact and disintegrated Mtb bacilli and mRNA in human tissue. (a) low power image of a human testicular specimen containing ZN-positive AFB. Inset–note positive ZN staining within the seminiferous tubules. (b) low power image of a consecutive section of the testicular specimen in (a) revealing RNAscope Mtb mRNA signals, which are abundant within the seminiferous tubules. Medium power image showing ZN-positive AFB (c) or RNAscope Mtb signals (d) within seminiferous tubules. High power image revealing ZN-positive AFB (e) and RNAscope Mtb mRNA signals (f). (g) High power image showing a spectrum of RNAscope Mtb mRNA signals within a single field (h–m). High power images of RNAscope signals from (h) intact bacilli, (i, j) partially intact bacilli, (k, l), disintegrated bacilli appearing as large, diffuse puncta (m) small puncta representing single extrabacillary Mtb mRNAs.