Figure 2.

The landscape of histone lactylation triggered by hypoxia in ESCC cells

(A) Global Kla level was increased in KYSE30 cells cultured in hypoxia environment.

(B) The experimental workflow of SILAC quantitative proteomics to analyze the landscape of histone Kla regulated by hypoxia in the in KYSE30 cells.

(C) Illustration of histone Kla sites identified in KYSE30 cells cultured in hypoxia environment.

(D) Scatterplot showing the ratio of Kla proteins in KYSE30 cells cultured in hypoxia versus normoxia.

(E) The Kla level of H4K16, H4K12, H4K8, H4K5, H3K18, and H3K9 in KYSE30 cells were increased in hypoxia.