Figure 5.

H3K9la influence the expression of LAMC2 in KYSE30 cells under hypoxia

(A) LAMC2 promoter in the genomic position was identified to enrich in H3K9la peaks when KYSE30 cells cultured in hypoxia.

(B) The ChIP-qPCR assays showed that H3K9la levels on LAMC2 promoter were significantly elevated in KYSE30 cells cultured in hypoxia (n = 3 per group).

(C) The qPCR assays monitoring expression of the LAMC2 in KYSE30 cells cultured in hypoxia (n = 4 per group).

(D) The ChIP-seq showed that H3K18la was not enriched in LAMC2 promoter in macrophage (GSE115354).

(E) The CUT&Tag-seq showed that LAMC2 promoter in the genomic position was identified to enrich in H4K12la peaks in microglia.

(F) The ChIP-qPCR assays showed that H3K18la can’t not enriched in LAMC2 promoter in KYSE30 cells cultured in hypoxia or normoxia (n = 3 per group).

(G) The ChIP-qPCR assays showed that H4K12la levels on LAMC2 promoter were not changed in KYSE30 cells cultured in hypoxia (n = 3 per group).

(H) Western blotting analysis of LAMC2 in KYSE30 cells in KYSE30 cells cultured in hypoxia or normoxia.

(I) TCGA-ESCC data showed that the expression of LAMC2 were related with HIF1-α.

(J) Sodium oxamate decreased the intracellular lactic acid in KYSE30 cells (n = 3 per group).

(K) The ChIP-qPCR assays showed that H3K9la levels on LAMC2 promoter were decreased in KYSE30 cells stimulated by sodium oxamate in hypoxia (n = 3 per group).

(L) The qPCR assays monitoring expression of the LAMC2 in KYSE30 cells stimulated by sodium oxamate in hypoxia (n = 3 per group).

(M) Pan Kla decreased in KYSE30 cells stimulated by sodium oxamate under hypoxia.

(N) H3K9la and LAMC2 were decreased in KYSE30 cells stimulated by sodium oxamate under hypoxia. Statistical significance was analyzed by Student’s t-test. Data are mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ns indicates no significance.