Figure 6.

LAMC2 plays an oncogenic role in ESCC

(A–C) TCGA, GSEA, GENT2 data showed that LAMC2 mRNA levels was highly expressed in ESCC tumors compared to the normal controls.

(D) CCK-8 assay showed that overexpress LAMC2 promoted the cell viability (n = 3 per group).

(E) Colony formation assay of KYSE30 cells with overexpression of LAMC2.

(F) Transwell assays show the migratory and invasive abilities of KYSE30 cells with overexpression of LAMC2. The scale bar shows 100 μm.

(G) Overexpressed LAMC2 and control KYSE30 cells were subcutaneously implanted into mice. The tumor sizes were continuously recorded to draw tumor growth curves. The scale bar shows 1 cm.

(H) The number of metastatic tumor foci in lung.

(I) Representative images of Ki-67 staining of tumor sections in xenograft mouse models. The scale bar shows 100 μm in figure 10 × and 50 μm in figure 20 ×.

(J) Hematoxylin-eosin staining of metastatic tumor foci in lung. The scale bar shows 200 μm.

(K) Volcano map presents that 1071 mRNAs were up-regulate and 937 mRNA were down-regulate in LAMC2 overexpressed KYSE30 cells (p adjust<0.05, |Log₂FC|>1).

(L) KEGG enrichment analysis of the DEGs.

(M) Expression of VEGFA, AKT and p-AKT in overexpressed LAMC2 KYSE30 cells. Statistical significance was analyzed by Student’s t-test. Data are mean ± SEM. ∗p < 0.05, ∗∗p < 0.01.