REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Alpha Tubulin Polyclonal antibody	Proteintech	Cat No. 11224-1-AP; RRID: AB_2210206
Anti-L-Lactyl-Histone H3 (Lys9) Rabbit mAb	PTMBIO	Cat: PTM-1419RM; RRID: AB_3076695
Anti-L-Lactyl-Histone H3 (Lys18) Rabbit mAb	PTMBIO	Cat: PTM-1406RM; RRID: AB_2909438
Anti-L-Lactyl-Histone H4 (Lys5) Rabbit pAb	PTMBIO	Cat: PTM-1407; RRID: AB_3096309
Anti-L-Lactyl-Histone H4 (Lys8) Rabbit mAb	PTMBIO	Cat: PTM-1415RM; RRID: AB_3101829
Anti-H4K12la Rabbit pAb	PTMBIO	Cat: PTM-1411; RRID: AB_2941896
Anti-L-Lactyl-Histone H4 (Lys16) Rabbit mAb	PTMBIO	Cat: PTM-1417RM; RRID: AB_3101830
Anti-Histone H3.3 Rabbit mAb	PTMBIO	Cat: PTM-7294; RRID: AB_3101831
Anti-L-Lactyl Lysine Rabbit pAb	PTMBIO	Cat: PTM-1401; RRID: AB_2868521
LAMC2 Polyclonal antibody	Proteintech	Cat No. 19698-1-AP; RRID: AB_10644139
VEGFA Polyclonal antibody	Proteintech	Cat No. 19003-1-AP; RRID: AB_2212657
DYKDDDDK tag Monoclonal antibody	Proteintech	Cat No. 66008-4-Ig; RRID: AB_2918475
HIF-1 alpha Polyclonal antibody	Proteintech	Cat No. 20960-1-AP; RRID: AB_10732601
LDHA Rabbit pAb	abclonal	Cat: A1146; RRID: AB_2758572
Ki67 Rabbit pAb	abclonal	Cat: A11390; RRID: AB_2758539
AKT Monoclonal antibody	Proteintech	Cat No. 60203-2-Ig; RRID: AB_10912803
Phospho-AKT (Ser473) Recombinant antibody	Proteintech	Cat No. 80455-1-RR; RRID: AB_2918892
Anti-L-lactyllysine antibody conjugated agarose beads	PTMBIO	Cat: PTM-1404; RRID: AB_3101832
Bacterial and virus strains
DH5α Competent Cells	Sangon Biotech	Cat: B528413-0100
Chemicals, peptides, and recombinant proteins
Trypsin	Beijin life proteomic	Cat: 9002-07-7
Deacetylase Inhibitor Cocktail 100 ×	Beyotime	Cat: P1113
Trizol	Invitrogen	Cat: 15596026
2 - NBDG	MedChemExpress	Cat: 186689-07-6
MNase	Merck	Cat: 9013-53-0
2 × RealStar Fast SYBR qPCR Mix	GenStar	Cat: A301-10
DMEM for SILAC	Thermo Scientific™	Cat: A33822
Fetal Bovine Serum Dialyzed	ThermoFisher	Cat: 30067334
L-Argnie- HCl	ThermoFisher	Cat: 88427
L-13C6-lysine-2 × HCl	ThermoFisher	Cat: 88431
L-lysine-2 × HCl	ThermoFisher	Cat: 88429
Fetal Bovine Serum	VivaCell	Cat: C2910-0500
Roswell Park Memorial Institute (RPMI) 1640	ThermoFisher	Cat: C3001-0500
Penicillin-Streptomycin Solution, 100×	Beyotime	Cat: C0222
Defined Keratinocyte-SFM a serum-free medium	Gibco	Cat: MEPI500CA
EpiLife	Gibco	Cat: 10744019
RIPA Lysis Buffer	Beyotime	Cat: P0013B
PMSF	Beyotime	Cat: ST505
C18 ZipTips	Millipore Corp	Cat: ZTC18M096
Critical commercial assays
Pierce™ BCA Protein Assay Kit	Thermo Scientific™	Cat: 23227
CheKine™ Micro Lactate Assay Kit	Abbkine	Cat: KTB1100
DAB Kit	Solarbio	Cat: DA1015
Anti-L-lactyllysine antibody conjugated agarose beads	PTM	PTM-1014K
Deposited data
MS/MS analysis for protein quantification	This paper	ProteomeXchange Consortium: PXD048995.
MNase ChIP-seq and RNA-seq data	This paper	Gene Expression Omnibus (GEO): GSE255352
Experimental models: Cell lines
KYSE 30	Pricella Life	Cat: CL-0577
KYSE 140	FengHuiShengWu	Cat: CL0437
KYSE 150	Pricella Life	Cat: CL-0638
KYSE 450	FengHuiShengWu	Cat: CL0606
NE3	BLUEFBIO™	Cat: BFN607200102
HEK-293T	ATCC	Cat: CRL-11268
Experimental models: Organisms/strains
BALB/c nude	Beijing Vital River Laboratory Animal Technology Co., Ltd	https://buy.vitalriver.com/commodity/detail?productId=402883af7e23dcd2017e23dd211e0adb
Oligonucleotides
LAMC2 ChIP3-For 5’ GTGGAGAGGACCCTGTTGTG 3’	tsingke	HPLC-purified
LAMC2 ChIP3-Rev 5’ CCAGGGCAGATTCACAGAGG 3’	tsingke	HPLC-purified
LAMC2 qPCR-For 5’ GACAAACTGGTAATGGATTCCGC 3’	tsingke	HPLC-purified
LAMC2 qPCR-Rev 5’ TTCTCTGTGCCGGTAAAAGCC 3’	tsingke	HPLC-purified
GAPDH-qPCR-For 5’ GCACCGTCAAGGCTGAGAAC 3’	tsingke	HPLC-purified
GAPDH-qPCR-Rev 5' TGGTGAAGACGCCAGTGGA 3'	tsingke	HPLC-purified
LAMC2-shRNA1 5’ GCCCTGCAATTGTAACTCCAA 3’	tsingke	HPLC-purified
LAMC2-shRNA2 5’ GCTCACCAAGACTTACACATT 3’	tsingke	HPLC-purified
5’ AGGGCAGAATCATCACGAAGT 3’ VEGFA-qPCR-For	tsingke	HPLC-purified
5’ AGGGTCTCGATTGGATGGCA 3’ VEGFA-qPCR-Rev	tsingke	HPLC-purified
Recombinant DNA
Plasmid: plvx-puro	This paper	N/A
Plasmid: Plko.1-puro	This paper	N/A
Software and algorithms
R	https://www.r-project.org	N/A
DESeq2	https://github.com/mikelove	N/A
GraphPad Prism 8	www.graphpad.com	N/A
MaxQuant (v.1.5.5.1)	https://www.maxquant.org/	N/A