Fig. 2.

ALD induces in significant changes in the lipidome. A: Volcano plot of lipid levels. The vertical axis contains the P-value (−log10) from Welch t-tests between ALD and controls, and the horizontal axis the fold change (log2) between ALD and controls. Colored dots are lipids with a P-value of <0.05. Each color represents a different lipid class. B: PCA analysis shows distinct group of ALD and control fibroblasts. C: Heatmap of Z-scores of top 50 lipids based on P-value sorted on lipid class. D: Box plot of the newborn screening marker LPC 26:0 in ALD and control fibroblasts (∗∗∗∗P < 0.0001). ALD, adrenoleukodystrophy; LPC, lysophosphatidylchloline; PCA, principal component analysis.