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. 2024 Jul 1;20(9):3638–3655. doi: 10.7150/ijbs.96173

Figure 1.

Figure 1

piR-4447944 is upregulated in CRPC tissues and cell lines. A, Small RNA sequencing revealed differentially expressed piRNAs among benign prostate tissues, HSPCa and paired CRPC tissues. The heatmap illustrated a distinguishable piRNA expression profiling among different groups. Red color indicates high expression and blue color indicates low expression. B, Box plot showed the expression levels of 117 significantly upregulated piRNAs in CRPC group, as compared with benign prostate group and HSPCa group. Each dot in one group indicated an individual piRNA and their expression levels were normalized to log2CPM (log2 counts per million). C, Expression levels of the top 10 highly expressed piRNAs in CRPC group were validated in a bigger cohort by RT-qPCR. Four piRNAs were validated to be significantly upregulated in the CRPC group compared to the HSPCa and benign prostate groups. D, The expression levels of two validated piRNAs in androgen independent LNCaP-AI cells were significantly higher than their parental hormone sensitive LNCaP cells. E, The expression levels of piR-4447944 and piR-4419185 were examined by qRT-PCR in human normal prostate epithelial cell line RWPE-1 and five human PCa cell lines (LNCaP, VCaP, 22Rv1, DU145 and PC3); data were normalized to RWPE-1 cells. Results are presented as mean ± SD. *p < 0.05; **p < 0.01, ***p < 0.001, ****p < 0.0001. Data were obtained from at least three independent experiments.