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. 2024 Jul 1;20(9):3638–3655. doi: 10.7150/ijbs.96173

Figure 2.

Figure 2

piR-4447944 promotes androgen-independent growth of PCa cells. A, Efficiency of piR-4447944 overexpression in PCa cells LNCaP and 22Rv1 by piRNA-mimic was verified by RT-qPCR. B, Effect of piR-4447944 overexpression on the cell viability of LNCaP and 22Rv1 cells under androgen-deprivation culture condition (CS-FBS) was determined by CCK8 assay. CS-FBS indicates charcoal-stripped fetal bovine serum, mimicking the androgen-deprivation conditions. C-D, The effect of piR-4447944 overexpression on EdU incorporation ability (C), colony formation ability (D) of LNCaP cells and 22Rv1 cells in androgen-deprived cultural medium. E, Cell cycle distribution of LNCaP and 22Rv1 cells transfected with NC-mimic or piR-4447944 mimic cultured in androgen-deprived cultural medium were detected by flow cytometry, and quantitative analysis of cell cycle distribution was shown in the bottom panel. The cell proliferation index was calculated as (S+G2/M)/(G0/G1+S+G2/M) x 100%. Results are presented as mean ± SD. *p < 0.05; **p < 0.01, ***p < 0.001, ****p < 0.0001. Data were obtained from at least three independent experiments.