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. 2024 Jul 1;20(9):3638–3655. doi: 10.7150/ijbs.96173

Figure 4.

Figure 4

piR-4447944 suppression restores castration sensitivity and inhibits migration and invasion of LNCaP-AI cells. A-B, Identification of LNCaP-AI cells by cell morphology changes (A, magnification, 40x) and western blot (B). C, Knockdown efficiency of piR-4447944 inhibitor in LNCaP-AI cells was verified by RT-qPCR. D-F, Effect of piR-4447944 knockdown on LNCaP-AI cells viability in the androgen-deprived cultural medium was determined by CCK-8 assay (D), EdU incorporation assay (E) and colony formation (F). G, Representative flow cytometric histograms of PI staining of LNCaP-AI cells with control or piR-4447944 knockdown. Percentages of LNCaP-AI cells in the G0/G1, S and G2/M phases were quantified and shown in the bottom. The cell proliferation index was calculated as (S+G2/M)/(G0/G1+S+G2/M) x 100%. H-I, Influence of piR-4447944 knockdown on LNCaP-AI cells migration and invasion ability was evaluated by Transwell assays (H) and wound-healing assay (I). Results are presented as mean ± SD. *p < 0.05; **p < 0.01, ***p < 0.001, ****p < 0.0001. Data were obtained from at least three independent experiments.