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. 2024 Jun 24;20(9):3570–3589. doi: 10.7150/ijbs.96671

Figure 3.

Figure 3

CircATIC sponged miR-1247-5p. A. TCGA database and CircInteractome database predicted 14 miRNAs candidates potentially binding to circATIC. B. RT-qPCR assessed the expression of miRNA candidates in BLca cell lines. C. TRAP assay confirmed that we obtained circATIC-MS2 complex comparing to the negative control (MS2). D. Western blotting showed that AGO2 was enriched on circATIC-MS2 complex. E. RT-qPCR showed that miR-1247-5p was significantly enriched on the circATIC-MS2 complex. F. miR-1247-5p expression in BLca tissues and normal tissues from TCGA database and the correlation between miR-1247-5p expression and PTBP1 expression in BLca tissues from TCGA database. G. miR-1247-5p expression in BLca tissues compared to normal tissues from our hospital. H. miR-1247-5p expression in BLca cells upon overexpression of miR-1247-5p. I. Luciferase activity showed that circATIC specifically sponged miR-1247-5p via the core binding sequence. J. FISH assay showed that miR-1247-5p was dominantly located in cytoplasm. K. EdU assay showed that decreased miR-1247-5p (miR inhibitor) rescued the proliferation inhibited by circATIC knockdown. L-M. Wound-healing assay and transwell assay showed that the inhibition of migration and invasion of BLca cells induced by circATIC knockdown was rescued by silencing of miR-1247-5p (miR inhibitor).