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. 2024 Jun 24;20(9):3570–3589. doi: 10.7150/ijbs.96671

Figure 4.

Figure 4

miR-1247-5p directly targeted RCC2. A. miRDB, starbase and Targetscan predicted the potential target genes of miR-1247-5p. B. The correlation between miR-1247-5p expression and RCC2 mRNA expression in BLca tissues from TCGA database. The correlation between PTBP1 mRNA expression and RCC2 mRNA expression in BLca tissues from TCGA database. C. The correlation between miR-1247-5p expression and RCC2 mRNA expression in BLca tissues from our hospital. D. The correlation between circATIC expression and RCC2 mRNA expression in BLca tissues from our hospital. E. Forced expression of miR-1247-5p induced down-regulation of RCC2 mRNA. F. CircATIC inhibition induced down-regulation of RCC2. G. Knockdown of circATIC inhibited RCC2 protein, while knockdown of miR-1247-5p (miR-inh) reversed this effect. H. Schematic diagram of wide-type dual-luciferase reporter vector (WT RCC2) and mutant (Mut RCC2) regarding the indicated site. I. Dual-luciferase reporter assay showed that miR-1247-5p bounded to the specific binding sequence on RCC2 3'UTR. J-K. RCC2 mRNA expression level in BLca cell lines and SV-HUC1 cell. L. RCC2 mRNA expression in BLca tissues from TCGA database. M. RCC2 mRNA expression in BLca tissues from our hospital. N. RCC2 mRNA expression in BLca tissues from our hospital. O. Western blotting assay showed the RCC2 protein level in BLca tissues (T) and paired normal tissues (N). P. IHC staining of RCC2 in BLca tissues and paired normal tissues.