Fig. 2.
Resistance of Hif-2afl mice persists in vitro. (A,B) Splenic cells from naïve Hif-1afl and Hif-2afl mice were isolated and cultivated as spleenoids. Spleenoids were infected with FV in vitro and analyzed at 4 dpi by flow cytometry (A). Viral load was determined by real time-PCR analysis (B). Expression of FV envelope (env) RNA was evaluated and normalized to β-actin expression and Hif-1afl. Results are mean±s.e.m. The numbers in the graphs indicate the number of animals tested. *P<0.05; ns, not statistically significant (two-tailed unpaired Student's t-test).