Fig. 4.

MHC I and protein kinase C are not involved in FV resistance of Hif -2a^fl/fl mice to FV infection. (A) Chromosomal structure of the murine chromosome 17. (B) The Mhc I haplotype of two individual mice from the Hif-1a^fl and Hif-2a^fl strains was determined by RT-PCR and compared to individuals from the FVB mouse strain as negative control. Image representative of one experimental repeat with two individual mice. (C) To determine whether FV infection alters Prkce expression, we performed mRNA expression analyses of naïve and FV-infected Hif-1a^fl mice (7 dpi) and spleenoids (4 dpi). (D) Spleenoids of Hif-1a^fl animals were treated with FV alone or in combination with 0.1 µM of the PRKC inhibitor GF109203X. At 4 dpi, the number of Ter119⁺ erythroblasts was determined by flow cytometry. Results are mean±s.e.m. The numbers in the graphs indicate the number of animals tested. *P<0.05; ns, not statistically significant (two-tailed unpaired Student's t-test).