FIG. 2.
Specific M protein-mRNA 1 interaction in MHV-infected cells. MHV-JHM-infected DBT cells were labeled with 32Pi from 6 to 8 h p.i. in the presence of actinomycin D, and cytoplasmic protein lysates were prepared. The intracellular (i.c.) proteins were immunoprecipitated with an anti-N protein monoclonal antibody (lane 2), an anti-M protein monoclonal antibody (lane 3), or an anti-H2K monoclonal antibody (lane 4). MHV-specific RNAs were extracted from the immunoprecipitated samples and analyzed by agarose-formaldehyde gel electrophoresis. Lane 1, virus-specific RNAs extracted from 32P-labeled MHV-infected cells at 8 h p.i.