Figure 1.

Schematic of experimental workflow to deliver mRNA encapsulated in LNPs. Adult and neonatal CD-1 mice were used for in vivo experiments. Intravitreal injections of mRNA LNPs were performed to deliver the reagents to the vitreous chamber. Ex vivo experiments were performed using human retinal explants or human RPE cultures. After histological processing, analysis was completed using immunofluorescence and confocal imaging to determine transfection cell type expression and duration.