Figure 2.

Intravitreal injections of LNP-packaged EGFP mRNA transfects retinal cells in the mouse. Two amino lipid formulations, 2T and 6T, were compared for their ability to transfect retinal cells at various time points. Confocal images of retinal cross-sections with DAPI and anti-GFP immunostaining are shown. EGFP is expressed in similar cell populations at (A) 48 hours, (B) 72 hours, (C) 96 hours, (D) 1 week, and (E) 2 weeks for both 2T and 6T LNPs. Images shown are representative of at least three retinas (scale bar = 50 µm).