Figure 8.

EGFP mRNA encapsulated in LNPs transfects adult human retina explants. Adult human post-mortem eye globes were treated with EGFP mRNA LNPs for 4 hours. (A) Confocal images of human retina cross-section treated with EGFP mRNA encapsulated in 6T LNPs and then stained with DAPI, anti-GFP, and anti-GFAP. GFP is expressed in cells with apical and basal processes. (B) Cross-section of human retina treated with EGFP mRNA encapsulated in 2T LNPs. GFP is expressed in perivascular cells surrounding a blood vessel, indicated by the arrow, and in cells with apical and basal processes. (C) Flat mount of parafoveal retina treated with EGFP mRNA in 6T LNPs. GFP is expressed in cells in the fovea. (D) Flat mount of retina with RPE and choroid treated with EGFP mRNA in 2T. Perivascular cells express GFP. (E) Flat mount of retina treated with 6T. Arrows indicate GFP positive cells along a small blood vessel. Images shown are representative of retinas and RPE from two globes from a single donor (scale bars = 50 µm).