Skip to main content
. 2024 Jul 10;10(28):eadk3365. doi: 10.1126/sciadv.adk3365

Fig. 4. Silencing Tac2 neurons in the CeM reduces the proportion of significant correlations of calcium traces with freezing in males but does not affect this proportion in females.

Fig. 4.

(A) Schematic timeline of the procedure. Created with BioRender.com. (B) Tac2-Cre−/− and Tac2-Cre−/+ males and females show no differences during FA. (C) The average freezing during the preCS and all the CS was measured in FE. Temporarily silencing Tac2 neurons in the CeM reduces memory consolidation in males and enhances it in females. (D) Heatmap representing the fluorescence of each neuron throughout the FE session in males. (E) Raw fluorescence in the lateral posterior BNST during the FE session in males. (F) Average raw fluorescence in males during the preCS, tone, and ITI. (G) Heatmap representing the fluorescence of each neuron throughout the FE session in females. (H) Raw fluorescence in the lateral posterior BNST (or STPL) during the FE session in females. (I) Average raw fluorescence in females during the preCS, tone, and ITI. (J) The proportion of neurons correlating with freezing in Tac2-Cre−/− and Tac2-Cre−/+ males during the tone in FE. (K) The proportion of neurons correlating with freezing in Tac2-Cre−/− and Tac2-Cre−/+ females during the tone in FE. Data are shown as mean ± SEM and analyzed using repeated measures ANOVA, Mann-Whitney’s U, or Pearson’s chi-square test. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.