TABLE 1.

Names and sequences of the oligonucleotides used for the generation of promoter mutants

Oligonucleotide namea	Sequenceb	Promoter(s)
5′ and 3′ deletions
5′-A	cgcgagatctGGGTCGCCGGAGAAAAGTCAAAAGG	p72, p72.1, p72.2
5′-B	cgcgagatCTAGCAAGAGCGTGTCAATAATT	p72.3, p72.5
5′-C	cgcgagaTCTTTGTTATTATCAAGATCC	p72.4
3′-A	gcgcggatccTATATAATGTTATAAAAATAATTT	p72, p72.3, p72.4
3′-B	gcgcggatccAATTATTGACACGCTCTTGCT	p72.1
3′-C	gcgcggatccGGATCTTGATAATAACAAAGA	p72.2, p72.5
6A	gatcTATTTAATAAAAACAATAAATTATTTTTATAACATTATATAg
6B	gatccTATATAATGTTATAAAAATAATTTATTGTTTTTATTAAATA	p72.6
7A	gatctTATTTTTATAACATTATATAg
7B	gatccTATATAATGTTATAAAAATAA	p72.7
Linker scan substitutions
5′-8	gcggtaccgcgcgcATAAAAACAATAAATTATTTTTATAACATTATATAggatccccccccc	p72.8
5′-9	gcggtaccTATTTAgcgcgAACAATAAATTATTTTTATAACATTATATAggatccccccccc	p72.9
5′-10	gcggtaccTATTTAATAAAgcgcgTAAATTATTTTTATAACATTATATAggatccccccccc	p72.10
5′-11	gcggtaccTATTTAATAAAAACAAgcgcgTATTTTTATAACATTATATAggatccccccccc	p72.11
5′-12	gcggtaccTATTTAATAAAAACAATAAATgcgcgTTATAACATTATATAggatccccccccc	p72.12
5′-13	gcggtaccTATTTAATAAAAACAATAAATTATTTgcgcgACATTATATAggatccccccccc	p72.13
5′-14	gcggtaccTATTTAATAAAAACAATAAATTATTTTTATAgcgcgATATAggatccccccccc	p72.14
5′-15	gcggtaccTATTTAATAAAAACAATAAATTATTTTTATAACATTgcgcgggatccccccccc	p72.15
3′-G	gggggggggatcc	From p72.8 to p72.15
Single-nucleotide substitutionsc
5′-D	cgcgggtaccTATTTAATAAAAACAATAAATTA	From p72.16A to p72.21T
3′-16	gcgcggatccTATATBATGTTATAAAAATAATTTATTGTTTTT	p72.16A, p72.16C, p72.16G
3′-17	gcgcggatccTATAVAATGTTATAAAAATAATTTATTGTTTTT	p72.17C, p72.17G, p72.17T
3′-18	gcgcggatccTATBTAATGTTATAAAAATAATTTATTGTTTTT	p72.18A, p72.18C, p72.18G
3′-19	gcgcggatccTAVATAATGTTATAAAAATAATTTATTGTTTTT	p72.19C, p72.19G, p72.19T
3′-20	gcgcggatccTBTATAATGTTATAAAAATAATTTATTGTTTTT	p72.20A, p72.20C, p72.20G
3′-21	gcgcggatccVATATAATGTTATAAAAATAATTTATTGTTTTT	p72.21C, p72.21G, p72.21T
TATA substitutions in p10, p11.5, and p54 promoters
5′-p11.5	cgcgggtaccTTTAAAATAAGCCATTTAAAGATTTAGAATTTA	p11.5, p11.5mut
3′-p11.5	gcgcggatccTATACATATAAATTCTAAATCTT	p11.5
3′-p11.5mut	gcgcggatccgcgcCATATAAATTCTAAATCTT	p11.5mut
5′-p10	cgcgggtaccATAATAAAAAATATTTTTTACTTTTTTTTCTTC	p10, p10mut
3′-p10	gcgcggatccTATATTATGAAGAAAAAAAAGTA	p10
3′-p10mut	gcgcggatccgcgcTTATGAAGAAAAAAAAGTA	p10mut
5′-p54	cgcgggtaccGTAATTTCATTGCGCCACAACATTTTTATATAT	p54, p54mut
3′-p54	gcgcggatccTATAAATAATATATAAAAATGTT	p54
3′-p54mut	gcgcggatccgcgcAATAATATATAAAAATGTT	p54mut

^aOligonucleotides are listed according to the type of mutagenesis for which each oligonucleotide was used. 

^bNonviral sequences are in lowercase letters, and recognition sequences for restriction enzymes BglII, BamHI, and KpnI are underlined. 

^cPositions indicated as B are C, G, or T residues, and positions indicated as V are A, C, or G residues.